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| Registros recuperados: 13 | |
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| Salin, Karine; Villasevil, Eugenia M.; Anderson, Graeme J.; Auer, Sonya K.; Selman, Colin; Hartley, Richard C.; Mullen, William; Chinopoulos, Christos; Metcalfe, Neil B.. |
| Many animals experience periods of food shortage in their natural environment. It has been hypothesised that the metabolic responses of animals to naturally‐occurring periods of food deprivation may have long‐term negative impacts on their subsequent life‐history. In particular, reductions in energy requirements in response to fasting may help preserve limited resources but potentially come at a cost of increased oxidative stress. However, little is known about this trade‐off since studies of energy metabolism are generally conducted separately from those of oxidative stress. Using a novel approach that combines measurements of mitochondrial function with in vivo levels of hydrogen peroxide (H2O2) in brown trout (Salmo trutta), we show here that fasting... |
| Tipo: Text |
Palavras-chave: High-resolution respirometry; In vivo; Liver atrophy; MitoB probe; Mitochondrial respiratory state. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56022/57540.pdf |
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| Killen, Shaun S.; Croft, Darren P.; Salin, Karine; Darden, Safi K.. |
| Sexual coercion of females by males is widespread across sexually reproducing species. It stems from a conflict of interest over reproduction and exerts selective pressure on both sexes. For females, there is often a significant energetic cost of exposure to male sexually coercive behaviours. Our understanding of the efficiency of female resistance to male sexually coercive behaviour is key to understanding how sexual conflict contributes to population level dynamics and ultimately to the evolution of sexually antagonistic traits. Overlooked within this context are plastic physiological responses of traits within the lifetime of females that could moderate the energetic cost imposed by coercive males. Here, we examined whether conflict over the frequency... |
| Tipo: Text |
Palavras-chave: Locomotion; Metabolic rate; Phenotypic plasticity; Sexual conflict; Teleost fish. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00414/52502/53313.pdf |
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| Auer, Sonya K.; Anderson, Graeme J.; Mckelvey, Simon; Bassar, Ronald D.; Mclennan, Darryl; Armstrong, John D.; Nislow, Keith H.; Downie, Helen K.; Mckelvey, Lynn; Morgan, Thomas A. J.; Salin, Karine; Orrell, Danielle L.; Gauthey, Alice; Reid, Thomas C.; Metcalfe, Neil B.. |
| Organisms can modify their surrounding environment, but whether these changes are large enough to feed back and alter their evolutionary trajectories is not well understood, particularly in wild populations. Here we show that nutrient pulses from decomposing Atlantic salmon (Salmo salar) parents alter selection pressures on their offspring with important consequences for their phenotypic and genetic diversity. We found a strong survival advantage to larger eggs and faster juvenile metabolic rates in streams lacking carcasses but not in streams containing this parental nutrient input. Differences in selection intensities led to significant phenotypic divergence in these two traits among stream types. Stronger selection in streams with low parental nutrient... |
| Tipo: Text |
Palavras-chave: Eco-evolutionary dynamics; Eco-evolutionary feedbacks; Natural selection; Niche construction; Salmo salar; Selection differential; Selection gradient. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00414/52586/53426.pdf |
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| Salin, Karine; Villasevil, Eugenia M.; Auer, Sonya K.; Anderson, Graeme J.; Selman, Colin; Metcalfe, Neil B.; Chinopoulos, Christos. |
| The use of tissue homogenate has greatly aided the study of the functioning of mitochondria. However, the amount of ATP produced per oxygen molecule consumed, that is, the effective P/O ratio, has never been measured directly in tissue homogenate. Here we combine and refine existing methods previously used in permeabilized cells and isolated mitochondria to simultaneously measure mitochondrial ATP production (JATP) and oxygen consumption (JO(2)) in tissue homogenate. A major improvement over existing methods is in the control of ATPases that otherwise interfere with the ATP assay: our modified technique facilitates simultaneous measurement of the rates of uncorrected ATP synthesis and of ATP hydrolysis, thus minimizing the amount of tissue and time needed.... |
| Tipo: Text |
Palavras-chave: ATPase; Fluorescence; Magnesium green; Oxidative phosphorylation; Oxygraph. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00414/52500/53300.pdf |
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| Hood, Wendy R.; Austad, Steven N.; Bize, Pierre; Jimenez, Ana Gabriela; Montooth, Kristi L.; Schulte, Patricia M.; Scott, Graham R.; Sokolova, Inna; Treberg, Jason R.; Salin, Karine. |
| Animals display tremendous variation in their rates of growth, reproductive output, and longevity. While the physiological and molecular mechanisms that underlie this variation remain poorly understood, the performance of the mitochondrion has emerged as a key player. Mitochondria not only impact the performance of eukaryotes via their capacity to produce ATP, but they also play a role in producing heat and reactive oxygen species and function as a major signalling hub for the cell. The papers included in this special issue emerged from a symposium titled “Inside the Black Box: The Mitochondrial Basis of Life-history Variation and Animal Performance”. Based on studies of diverse animal taxa, three distinct themes emerged from these papers. 1) When linking... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56023/57543.pdf |
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| Salin, Karine; Villasevil, Eugenia M.; Anderson, Graeme J.; Selman, Colin; Chinopoulos, Christos; Metcalfe, Neil B.. |
| Mitochondrial efficiency is typically taken to represent an animal’s capacity to convert its resources into ATP. However, the term mitochondrial efficiency, as currently used in the literature, can be calculated as either the respiratory control ratio, RCR (ratio of mitochondrial respiration supporting ATP synthesis to that required to offset the proton leak) or as the amount of ATP generated per unit of oxygen consumed, ATP/O ratio. The question of how flexibility in mitochondrial energy properties (i.e. in rates of respiration to support ATP synthesis and offset proton leak, and in the rate of ATP synthesis) affects these indices of mitochondrial efficiency has tended to be overlooked. Furthermore, little is known of whether the RCR and ATP/O ratio vary... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56014/57529.pdf |
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| Salin, Karine; Auer, Sonya K.; Villasevil, Eugenia M.; Anderson, Graeme J.; Cairns, Andrew G.; Mullen, William; Hartley, Richard C.; Metcalfe, Neil B.. |
| In recent years evolutionary ecologists have become increasingly interested in the effects of reactive oxygen species (ROS) on the life-histories of animals. ROS levels have mostly been inferred indirectly due to the limitations of estimating ROS from in vitro methods. However, measuring ROS (hydrogen peroxide, H2O2) content in vivo is now possible using the MitoB probe. Here, we extend and refine the MitoB method to make it suitable for ecological studies of oxidative stress using the brown trout Salmo trutta as model. The MitoB method allows an evaluation of H2O2 levels in living organisms over a timescale from hours to days. The method is flexible with regard to the duration of exposure and initial concentration of the MitoB probe, and there is no... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00413/52494/53297.pdf |
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| Salin, Karine; Auer, Sonya K.; Rudolf, Agata M.; Anderson, Graeme J.; Selman, Colin; Metcalfe, Neil B.. |
| Standard metabolic rate (SMR) and maximum metabolic rate (MMR) typically vary two-or threefold among conspecifics, with both traits assumed to significantly impact fitness. However, the underlying mechanisms that determine such intraspecific variation are not well understood. We examined the influence of mitochondrial properties on intraspecific variation in SMR and MMR and hypothesized that if SMR supports the cost of maintaining the metabolic machinery required for MMR, then the mitochondrial properties underlying these traits should be shared. Mitochondrial respiratory capacity (leak and phosphorylating respiration) and mitochondrial content (cytochrome c oxidase activity) were determined in the liver and white muscle of brown trout Salmo trutta of... |
| Tipo: Text |
Palavras-chave: Brown trout; Fish; Liver; Oxygen consumption; White muscle. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00413/52497/53321.pdf |
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| Salin, Karine; Auer, Sonya K.; Rey, Benjamin; Selman, Colin; Metcalfe, Neil B.. |
| It is often assumed that an animal's metabolic rate can be estimated through measuring the whole-organism oxygen consumption rate. However, oxygen consumption alone is unlikely to be a sufficient marker of energy metabolism in many situations. This is due to the inherent variability in the link between oxidation and phosphorylation; that is, the amount of adenosine triphosphate (ATP) generated per molecule of oxygen consumed by mitochondria (P/O ratio). In this article, we describe how the P/O ratio can vary within and among individuals, and in response to a number of environmental parameters, including diet and temperature. As the P/O ratio affects the efficiency of cellular energy production, its variability may have significant consequences for animal... |
| Tipo: Text |
Palavras-chave: Mitochondrial coupling efficiency; Life history; Oxidative stress; Reactive oxygen species; Trade-off; Uncoupling. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00414/52509/53322.pdf |
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| Registros recuperados: 13 | |
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